Restriction Enzymes
Restriction Enzymes
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Risultati della ricerca filtrata
Thermo Scientific™ Buffer Set for Restriction Enzymes
Ensure the optimum reaction conditions for each restriction enzyme with this five buffer system.
Thermo Scientific™ SdaI Buffer (10X)
Optimize digestions with SdaI restriction enzyme using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ BseXI Buffer (10X)
Optimize digestions with BseXI restriction enzyme using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ Cfr10I Buffer (10X)
Optimize digestions with Cfr10I restriction enzyme using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ EcoRI Buffer (10X)
Optimize digestions with EcoRI restriction enzyme using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ Eco52I Buffer (10X)
Optimize digestions with Eco52I restriction enzyme using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ Cfr9I Buffer (10X)
Optimize digestions with Cfr9I restriction enzyme using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ BfuI Buffer (10X)
Optimize digestions with BfuI restriction enzyme using this buffer, premixed with BSA for enhanced stability.
Thermo Scientific™ FastDigest SpeI (BcuI)
FastDigest SpeI (BcuI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: AhlI).
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Per saperne di più
Sensitive to Heat Inactivation | No |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Bcu I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest SacI
The FastDigest SacI restriction enzyme recognizes GAGCT^C sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: Eco53kI, EcoICRI, Psp124BI, SstI).
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | SacI |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 3' protruding ends |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest NheI
Thermo Scientific FastDigest NheI restriction enzyme recognizes G^CTAGC site and cuts best at 37°C in 5–15 minutes using universal FastDigest Buffer.
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Nhe I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ BcuI (SpeI)
Cut at A^CTAGT sites with BcuI (SpeI) restriction enzyme, which performs best at 37°C in Tango buffer (Isoschizomers: AhlI, SpeI).
Product Type | Restriction Enzyme |
---|---|
Enzyme | BcuI (SpeI) |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X Buffer Tango |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Concentrazione | 10 U/μL |
Thermo Scientific™ FastDigest Eco31I (IIs class)
The FastDigest Eco31I restriction enzyme recognizes GGTCTC(1/5)^ sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: BsaI, BspTNI)
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Eco31I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not CpG Methylation-Sensitive,Not Dam Methylation-Sensitive |
Research Category | Scarless Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | Yes |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest™ NotI
The FastDigest NotI restriction enzyme recognizes GC^GGCCGC sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: CciNI).
Gli ordini eseguiti prima delle 14:00 saranno spediti oggi stesso. Gli ordini eseguiti dopo le 14:00 saranno spediti domani.
Per saperne di più
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Not I |
Methylation Sensitivity | CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 80°C |
Product Line | FastDigest™ |
Thermo Scientific™ FastDigest NdeI
The FastDigest NdeI restriction enzyme recognizes CA^TATG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (Isoschizomers: FauNDI).
Gli ordini eseguiti prima delle 14:00 saranno spediti oggi stesso. Gli ordini eseguiti dopo le 14:00 saranno spediti domani.
Per saperne di più
Sensitive to Heat Inactivation | Yes |
---|---|
Product Type | Restriction Enzyme |
Enzyme | Nde I |
Methylation Sensitivity | Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive,Not CpG Methylation-Sensitive |
Research Category | Traditional Cloning |
Compatible Buffer | 10X FastDigest Buffer/FastDigest Green Buffer |
Type IIS RE | No |
Optimal Reaction Temperature | 37°C |
Sporgenza | 5' protruding ends |
Inactivation Temperature | 65°C |
Product Line | FastDigest™ |